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Antioxidant properties of hydrolyzate produced from the crude extract of Hilsa kelee

Authors:

L. S. Meepegamage,

Uva Wellassa University, Badulla 90000, LK
About L. S.
Department of Animal Science
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K. P. U. T. Egodauyana,

Chinese Academy of Science, CN
About K. P. U. T.
South China Sea Institute of Oceanology
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E. D. N. S. Abeyrathne

Uva Wellassa University, Badulla 90000, LK
About E. D. N. S.
Department of Animal Science
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Abstract

Hilsa kelee also known as "Karattaya" is a seasonal fish mainly found in tropical coastal waters and used for fish feed production due to its low market value. Therefore, this study was designed to determine the functional properties of fish protein hydrolyzate (FPH) produced from the crude extract of Hilsa kelee. For extraction, 20 mg/mL concentrated water-soluble and salt-soluble crude extracts were mixed with protease, papain, α-chymotrypsin, and elastase in 1:100 (enzyme: substrate) ratios. Each sample was incubated at different time intervals (0, 3, 6, 9, 12, and 24 hours) under predetermined optimum pH and temperatures. SDS-PAGE, TBARS, and DPPH assays and ferrous chelation activity were measured in the selected best hydrolysates. According to SDS-PAGE analysis for water and salt extracted, the following hydrolysis time lags were selected, protease (3 h), α-chymotrypsin (6 h), Elastase (6 h)and papain (6 h for water and 3 h for salt extraction). In the TBARS assay, the FPHs fromsalt soluble proteins with papain showed the highest antioxidant properties (5.84±0.05 MDAmg/L) compared to other treatments (p<0.05). The DPPH assay showed the highest radicalscavenging activity with salt soluble proteins hydrolyzed with α-chymotrypsin (p<0.05).Analysis of ferrous chelation activity showed that FPHs produced from water-soluble proteinswith α-chymotrypsin (20.81±0.02%) had the highest chelation (p<0.05). This concludes, FPHsproduced from Hilsa kelee using papain and α-chymotrypsin enzymes contain antioxidantproperties, and iron-binding activity.

How to Cite: Meepegamage, L.S., Egodauyana, K.P.U.T. and Abeyrathne, E.D.N.S., 2022. Antioxidant properties of hydrolyzate produced from the crude extract of Hilsa kelee. AGRIEAST: Journal of Agricultural Sciences, 16(2), pp.13–24. DOI: http://doi.org/10.4038/agrieast.v16i2.113
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Published on 21 Dec 2022.
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